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. 2011 Jan 24;108(6):2605–2610. doi: 10.1073/pnas.1015788108

Fig. 3.

Fig. 3.

Gq-coupled receptor activation regulates TREK1 currents and membrane association of EGFP-CtTREK1 constructs. (A and B) Effect of activation of 5HTR2bR (A) or mGluRI on TREK1 expressed in Xenopus oocytes. Currents were elicited by voltage ramp (from −150 to +50 mV, 1 s in duration). (C and D) Representative example of EGFP-CtTREK1 fluorescence decrease induced by 5HT2cR (C) and mGluRI (D) in oocytes. (E and F) Representative example of TREK1 current inhibition induced by 5HT2cR (E) and mGluRI (F) in oocytes. Effect of 5HT2cR and mGluRI activation on TREK1 current and on membrane fluorescence of EGFP-CtTREK1. (G) Percentage of inhibition (black) or of fluorescence decrease (gray) induced by 5HT2cR and mGluRI activation. Inhibitions and fluorescence decreases are statistically significant (P < 0.001) for both 5HT2cR and mGluRI. (H) Half current inhibition time (black) or the half decrease time of fluorescence induced by 5HT2cR and mGluRI activation (gray). The numbers of cells tested are indicated in parentheses.