Fig. 3.
Atlastin-1 oligomerization in solution. SEC-MALS data are shown for wild-type atlastin-1 (residues 1–446) in its apo-state (red) or bound to GppNHp (green), GDP (purple), or GDP•AlFx (orange). The signal from the 90°-scattering detector and refractive index detector are shown as solid, colored lines and black dashed lines, respectively (Left, Y axis). Average molecular weight as calculated every second across the protein elution peak is shown as black circles (Right, Y axis). Theoretical molecular weights based on primary sequence for the monomer and dimer are indicated as horizontal, dashed lines. Proteins (20 μM) were incubated with nucleotides (2 mM) prior to SEC-MALS analysis.