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SRM of endogenous proteins from a colorectal tumor obtained at surgery. (A) Integrated intensities of the exogenously added mutant peptide and the endogenous mutant peptide from the tumor, as indicated. The integrated intensities correspond to the sum of the peak areas of the transitions described in Table S1, which are shown in B for the endogenous peptide. The asterisk indicates the heavy isotope (13C6/15N2) labeled lysine.
