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. 2011 Jan 24;108(6):2581–2586. doi: 10.1073/pnas.1018275108

Fig. 1.

Fig. 1.

Adult zebrafish display an innate light avoidance behavior. (A) Photograph of the L/D choice chamber. (B) Choice indices in the choice chamber during first, second, fifth, and all 5 min analyzed (a choice index of 1.0 represents 100% time spent on the dark side, and an index of −1.0 represents 100% time spent on the light side) (n = 12 for each group). (C) Scatter plot of B, showing the distribution of choice indices in the tested animals. (D) Choice indices of three trials with a 24-h interval between each trial. The means for choice indices are day 1 (0.58 ± 0.15), day 2 (0.66 ± 0.12), and day 3 (0.65 ± 0.09) (n = 13; P = 0.64, ANOVA followed by the Bonferroni post hoc test). (E) Schemes of different choice chambers. (F) Choice indices in different choice chambers (L/D, n = 26; light/gray, n = 23; gray/dark, n = 24; F = 28.55, ***P < 0.001 compared with gray/dark, Tukey's test). (G) Scheme of a visual acuity chamber for testing the ability of zebrafish to discriminate between gray and dark. (H) Comparable visual discrimination of gray segment over dark background (n = 6) vs. dark segment over light background (n = 5), as indicated by comparable % responses (F = 0.36, P = 0.56, Tukey's test).