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. 2011 Jan 24;108(6):2384–2389. doi: 10.1073/pnas.1019547108

Fig. 1.

Fig. 1.

α-Langerin, α-DEC205, and α-Clec9A are captured by CD8+ DCs in vivo, whereas α-DCIR2 is taken up by CD8− DCs. C×B6 F1 mice (A) or C57BL/6 mice (B) were inoculated i.p. with 5 or 10 μg of each Alexa 647-labeled α-Langerin, α-DEC205, α-Clec9A, and α-DCIR2 mAb. GL117 mAb labeled with Alexa 647 (blue histograms) was used as a control mAb. Data are plotted with the higher dose of GL117 because we did not see differences between inoculation of 5 or 10 μg. When available, we also added KO mice in the C57BL/6 background as an additional control (DEC205 KO and Langerin KO mice, gray histograms). Uptake of labeled mAb by splenocytes was evaluated 3 h after inoculation by multicolor flow cytometry (Fig. S1) in CD11chighCD8α+ DCs (Fig. S1, population C), CD8α− DCs (Fig. S1, population B), and PDCA-1+ PDCs (Fig. S1, population D). One experiment representative of two to three with similar results is shown.