Fig. 3.

α-Langerin-, α-DEC205-, and α-Clec9A–targeted HIV gag-p24 requires α-CD40 for in vivo cross-presentation by DCs to primed gag-specific CD8⁺ T cells. (A) Schematic for the design of the adoptive transfer experiment (Materials and Methods). (B) Thy 1.1 mice transferred 1 d before with CFSE-labeled Thy 1.2⁺ adenovirus-primed T cells were challenged with 25 μg of α-CD40 alone, 5 μg of α-DEC-gag-p24 alone, medium, or a combination of 5 μg of α-DEC-gag-p24 and 25 μg of α-CD40. Four days after challenge, IFN-γ production and proliferation of transferred Thy 1.2⁺ CD3⁺CD8⁺ T cells were assessed in a 6-h in vitro restimulation assay. (C) As in B, but adoptively transferred, gag-primed, enriched T cells were challenged with 25 μg of α-CD40 in combination with 5 μg of α-DEC205-, α-Langerin-, α-DCIR2-, α-Clec9A-, or control Ig-gag-p24 mAbs. In all cases, one experiment of two with similar results is shown.