Fig. 4.

Enhanced gag-specific immunity by prime/boost immunization. (A) C57BL/6 mice were primed and boosted 4 wk apart with 5 μg of α-receptor mAbs or nontargeted gag-p24 protein in the presence of 50 μg of poly IC or poly ICLC as an adjuvant (Adj). IFN-γ production was evaluated by intracellular cytokine staining 2 wk after boost in the CD3⁺CD4⁺ gated cells in response to reactive HIV gag-p24 or nonreactive HIV gag-p17 peptide mix. (B) As in A, but mean ± SD from three to five experiments with three to four mice per group are shown.