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. Author manuscript; available in PMC: 2011 Feb 14.
Published in final edited form as: Osteoarthritis Cartilage. 2006 Sep;14(9):849–858. doi: 10.1016/j.joca.2006.02.009

Fig. 4.

Fig. 4

Recombinant human HYAL-2 expressed in embryonic chick chondrocytes is enzymatically active. Following transfection of embryonic chick chondrocytes with V5-HYAL-2 plasmid, cell lysates were isolated and analyzed by HA zymography. Lane 1: cells transfected with empty pTracer-V5His vector alone; lane 2: cells transfected with V5-HYAL-2 plasmid alone; lane 3: cells co-transfected with V5-HYAL-2 together with a reverse-orientation (antisense) full-length HYAL-2 containing plasmid; lane 4: cells transfected with V5-HYAL-2 followed by subsequent transfection with HYAL-2 antisense morpholino oligonucleotides. Clear bands on a darkened background depict areas of hyaluronidase activity in which Alcian blue stainable HA has been degraded and released from the gel.