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. Author manuscript; available in PMC: 2011 Feb 14.
Published in final edited form as: Osteoarthritis Cartilage. 2006 Sep;14(9):849–858. doi: 10.1016/j.joca.2006.02.009

Fig. 7.

Fig. 7

Recombinant c-myc-HYAL-2 protein expressed by HeLa and COS-7 cells is not depleted by PI-PLC treatment of the cells. Replicate cultures of HeLa cells (panel A) and COS-7 cells (panel B) transiently transfected with c-myc-HYAL-2 plasmid were then treated with PI-PLC for varying times and the supernatant media collected (Supernatants). The remaining cells were then washed and extracted with lysis buffer (Cell Lysates). Controls included nontransfected cells (lane C) and cells transfected with empty pSecTag2 vector (lane V); lanes 0′, 30′, 60′, 120′, c-myc-HYAL-2 transfected cells incubated PI-PLC for 0, 30, 60 or 120 min. Std, molecular mass standard proteins.