Skip to main content
. Author manuscript; available in PMC: 2011 Feb 14.
Published in final edited form as: Leukemia. 2010 Jan 28;24(4):813–820. doi: 10.1038/leu.2009.302

Figure 4.

Figure 4

Re-emergence of tyrosine kinase activity after 8093 cells develop resistance to dasatinib. 8093 cells were grown in the presence of MEFs with 0.5 nM dasatinib. Fresh dasatinib was added every second day with the change of medium. 8093 cell lysates were prepared at different time points as indicated. The levels of PY20, p-Src Tyr416, Crkl, p-Stat5 and p-ERK1/2 were determined by Western blot analysis. Blots were stripped and re-probed with Bcr (N-20), Src and GAPDH antibodies to ensure equal loading and transfer of protein.