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Folding free energy distribution of the human prion gene. (A) Distribution of the E values of the shuffled sequences from the fourth 200 nt window encompassing nucleotides 150–350. The red bold line represents the E value of the fourth window in the native sequence. (B) ΔE of the 45 windows. The ΔE values of windows in the coding sequence, nucleotides 50–811, are shown in the gray area. The ΔE peak of the fourth window is circled in blue.
