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. 2011 Feb 14;6(2):e16611. doi: 10.1371/journal.pone.0016611

Figure 1. Preparation of CL4-displaying BV.

Figure 1

A) Immunoblot analysis. Wild-BV and CL4-BV (0.1 µg/lane) were subjected to SDS-PAGE, followed by immunoblot analysis with anti-CL4 antibody. The lysate of CL4-expressing L (CL4/L) cells was used as a positive control. B, C) Interaction of a CL4 binder with CL4-BV. Immunotubes were coated with the wild-BV or CL4-BV, and C-CPE (B) or mutated C-CPE (C) was added to the BV-coated immunotubes at the indicated concentration. C-CPE bound to the BV-coated tubes was detected by ELISA with an anti-his-tag antibody.