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. 2011 Feb 14;6(2):e14674. doi: 10.1371/journal.pone.0014674

Figure 4. Thermal denaturation of SoPIP2;1.

Figure 4

It can be visualized by SDS-PAGE since large aggregates are formed that cannot enter the gel. After incubation at room temperature the normal bands corresponding to monomeric, dimeric, trimeric and tetrameric SoPIP2;1 are evident. No aggregates were found at 50°C whereas at the melting point, 58°C, as determined by CD, part of the protein was aggregated. At 65°C the aggregation was complete. The samples were incubated at the indicated temperatures (25–65°C) for 10 minutes before sample loading buffer was added followed by 10 minutes incubation at room temperature. St, standard with indicated Mw in kDa in the left side of the gel.