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Samples run on a reducing 12.5% gel, and stained for total protein with Coomassie brilliant blue. Lane 1: Bio-Rad Precision Plus Protein Ladder; Lane 2: Whole cell lysate of non-expressing cells; Lane 3: Whole cell lysate of induced cells; Lane 4: IMAC purified A53C-his; Lane 5: Crude A53C-his PEGylation reaction product; Lane 6: Size exclusion FPLC purified A53C-his-PEG; Lane 7: IMAC purified WT-his; Lane 8: FPLC purified native WT.
