Figure 6. Disruption of mucoid P. aeruginosa biofilms.

Adherent biofilms of a mucoid clinical isolate were established in 96-well plates and subsequently treated with 1 mg/ml enzyme for 1 hour. Remaining biofilm was then quantified using an alginate-sensitive lectin-HRP conjugate and ABTS substrate. Signals were normalized to a buffer only treatment. Both enzymes removed a significant proportion of biofilm relative to the buffer control (p<0.01). Importantly, theA53C-his-PEG enzyme removed >15% more biofilm than the WT-his enzyme (p<0.025). Error bars represent standard deviation.