. 2011 Feb 14;6(2):e17042. doi: 10.1371/journal.pone.0017042

Table 1. Kinetic parameters for alginate degradation.

Enzyme	V_max[ΔA₂₃₅ (min · mg)⁻¹]	K_m(µg/ml)	V_max/K_m
WT-his	440±30	80±20	6±1
A53C-his	280±30	30±6	9±2
S32C-his-PEG	330±20	26±6	13±3
A41C-his-PEG	134±3	7.1±0.8	18±2
A53C-his-PEG	460±50	40±10	13±4
A270C-his-PEG	300±20	15±5	20±7
A328C-his-PEG	180±10	13±4	14±4

Depolymerization of BSWA was followed by monitoring absorbance at 235 nm. Kinetic parameters were determined by nonlinear regression of initial rate vs. substrate concentration data (Prism version 4.0).