Figure 1. Experimental setup for “infection” with T4P-coated beads.
A) Sketch of the setup. Beads were trapped and placed onto the surface of Hec1B cells. When the center of the laser trap is actively moved by x, the deflection of the bead from the center of the trap d is proportional to the force F. B) Green line: The laser focus was moved by x = ±0.5 µm with respect to the initial position with a frequency of 10 Hz. Black line: Typical signal of the quadrant photodiode showing the position d. Deflection from 0 verifies binding of the bead to the surfaces of the epithelial cell.