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. 2011 Feb 14;6(2):e17097. doi: 10.1371/journal.pone.0017097

Figure 4. The RING domain fusion protein alteres the Grk distribution.

Figure 4

(A) The schematic structure shows the constructions of E3 fusion proteins. RING-SH2Drk, the RING finger domain of D-CblL (red orthogon) fused with the SH2 domain of Drk (yellow orthogon). RING-PR, the RING finger domain was fused with PR motif (amino acid 638 to 643; PPLPPR; gray orthogon). RING was composed with the RING finger domain and the linker (blue orthogon) of D-CblL. The RING, RING-PR and RING-SH2Drk were in-frame fused with a Flag-tag (red circle). (B-F) Gurken is asymmetrically expressed (green) in the dorsal-anterior corner of the middle-stage wild-type egg chamber (B). The normal Gurken expression pattern spreads about 8 to 10 follicle cells (indicated by white line in B). Expression of D-CblL (C), RING-PR (E), or RING-SH2Drk (F) led to a reduced Grk distribution (white lines shown in C, E, F), but no significant effect was observed in the egg chamber expressing RING (D). (G) Quantitative data of B, C, D, E, and F, and the percentages indicated middle stage egg chambers with a normal pattern (*p<0.05; ** p<0.001).