Figure 2. PiCdc14 association with flagellar basal body complexes.

(A) FBBC from strain expressing PiCdc14/GFP, showing the protein in basal bodies (b), flagella (f), and nuclei (n). (B) Detection of PiCdc14/StrepTag in purified FBBCs and whole zoospores, using equal amounts of protein per lane and anti-StrepTag. (C, D) Colocalization in zoospores of PiCdc14/GFP (green) with flagella (pink, stained with anti-β-tubulin). Basal bodies and selected flagella are indicated. Bars represent 2 µm.