Table 2.
Pathotyping and replication of the H10N8 virus in chickens§
| Infection route | Days of post infection | Virus isolated from swabs | No.of Survivors | No.of Seroconverted Chickensb | HI titers (Log2) | |||
|---|---|---|---|---|---|---|---|---|
| Oropharyngeal | Cloacal | |||||||
| No.of Chickens shedding virus | Titera (log10 EID50/ml) | No.of Chickens shedding virus | Titera (log10 EID50/ml) | |||||
| Intravenous (8) | 3 | 3 | 1.7 ± 0.3 | 4 | 3.1 ± 0.7 | 8 | 8 | 6.3 ± 0.5 |
| 5 | 7 | 3.8 ± 0.6 | 4 | 3.0 ± 0.7 | ||||
| 7 | 5 | 1.7 ± 0.5 | 3 | 1.6 ± 0.5 | ||||
| Intranasal(8) | 3 | 5 | 2.3 ± 0.8 | 4 | 2.0 ± 0.3 | 8 | 8 | 5.6 ± 1.2 |
| 5 | 8 | 3.4 ± 1.1 | 7 | 3.3 ± 0.1 | ||||
| 7 | 6 | 2.5 ± 0.9 | 5 | 1.3 ± 0.4 | ||||
§One group of 8 six-week-old specific-pathogen-free white leghorn chickens were inoculated with 0.2 ml of 1:10diluted stock virus (106.3 EID50) intravenously and another group were inoculated with 106.0 EID50 of the virus in a 0.1 ml volume intranasally, and observed for 2 weeks after infection.
a The mean titer in EID50/ml of swab media of the positive chickens.
b Sera were harvested 3 weeks after infection, and seroconversion was confirmed by HI test.