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Labeling of tight junctional proteins in conjunctiva. Immunofluorescent staining of ZO-1 (red) and E-cadherins (green) in tissue sections from five treatment groups. A) No sensitization, no challenge (naïve animals); B) Sensitized, challenged; C) Sensitized, challenged, drug vehicle only; D) Sensitized, challenged, 0.1% topical olopatadine; E) Sensitized, challenged, 0.025% topical alcaftadine. Note decreased staining in sensitized, challenged animals without drug treatment (B and C).
