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. 2011 Feb 1;124(5):699–705. doi: 10.1242/jcs.078964

Fig. 3.

Fig. 3.

Immunofluorescence confocal micrographs showing protein localization in wild-type yw embryos. (A–G) Embryos were probed with non-muscle myosin (A,D), muscle myosin (B,E), and dUNC-45 (C,F,G) antibodies. (A–C) 2-hour-old embryos. At this stage, both non-muscle myosin and dUNC-45 localized to the embryonic blastoderm (yellow stars). Furthermore, UNC-45 can be seen in the pole cells (upper left of C). Muscle myosin is not detected in 2-hour-old embryos (B). (D–F) Ventral views of 14-hour-old embryos. At 14 hours, dUNC-45 localization is more similar to that of muscle myosin, which can be found in the body wall (pink arrowheads) and the pharyngeal muscles (white diamonds). G gives a lateral view of an embryo, confirming dUNC-45 localization in the body-wall muscle. Scale bars: 75 μm.