Fig. 5.

Western blot analysis of dUNC-45 and myosin expression in wild-type (yw), Tom34^EY03034 and T-33 22-hour-old embryos. (A) Western blot analysis of dUNC-45 expression. Control is bacterially expressed recombinant dUNC-45. (B) Western blot analysis of myosin expression. Control is indirect flight muscle lysate. Results of the analysis show that dUNC-45 can still be detected in the Tom34^EY03034 line but not in the T-33 line. Muscle myosin heavy chain is somewhat reduced in Tom34^EY03034 homozygotes, but is nearly absent from T-33 embryos. 10 μg of protein were loaded for each sample.