Fig. 5.

SPTV and LDCV proteins are accumulated in a TGN subcompartment that contains Golgin97 and some TGN46, but no TGN38 or calnexin (ER) after 20°C block. (A) The location of the trans-Golgi marker TGN38 (red) tagged with HA visualized by anti-HA polyclonal antibody was compared with that of VAChT–GFP during temperature block and release. The average OCC ± s.e.m. of VAChT–GFP with respect to TfnR was calculated from three different experiments (n=30). SYN–GFP- or CgA–RFP-expressing cells were incubated at 20°C and labeled with primary antibodies against calnexin (ER) (B), Golgin97 (TGN) (C) and TGN46 (D). Scale bars: 5 μm.