Fig. 6.

A pool of SPTV proteins, but not LDCV proteins, remains unchanged in recycling endosomes during 20°C block and release. (A) The recycling endosomes were marked with the monoclonal antibody against transferrin receptor (TfnR, red) in cells expressing VAChT–GFP during 20°C block and release. The average OCC ± s.e.m. of VAChT–GFP with respect to TfnR was calculated from three different experiments (n=30). Note that cells of interest are indicated by arrowheads. SYN–GFP- or CgA–RFP-expressing cells were incubated at 20°C and labeled with primary antibodies against EEA1 (EEA, early endosomes) (B) and GGA (C). Scale bars: 5 μm.