Fig. 7.

The TGN subcompartment containing SYN–GFP and CgA–RFP recruits the subunits of SNARE complex after 20°C block. (A) SYN–GFP-expressing cells were incubated at 20°C and labeled with antibody against endogenous CgA (eCgA). (B) CgA–RFP-expressing cells incubated at 20°C are labeled with antibody against endogenous synaptophysin (eSYN). Cells expressing either SYN–GFP or CgA–RFP are incubated at 20°C and labeled with primary antibodies against VAMP4 (C), SNAP25 (D) or syntaxin-1 (E). Scale bars: 5 μm.