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Cortical neurons show colocalization of SYN–GFP and CGA–RFP after cold temperature block at 20°C. (A) After cold temperature block, SYN–GFP and CGA–RFP colocalize in the membranous compartment of cortical neurons. (B) The colocalization of SYN–GFP and CGA–RFP is not detectable at steady state at 37°C. (C) The average OCC between SYN–GFP and CGA–RFP in cortical neurons is higher at 20°C than at 37°C (*P<0.05). Scale bars: 5 μm.
