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. Author manuscript; available in PMC: 2012 Feb 15.
Published in final edited form as: J Immunol. 2011 Jan 14;186(4):2080–2086. doi: 10.4049/jimmunol.1001869

FIGURE 5.

FIGURE 5

CMV-tetramer–binding CD8+ T cells expand in response to CpG-activated naive B cells in the presence of CMV Ags. Naive B cells (nB) from five CMV seropositive donors were treated with (CpG-nB) or without CpG ODN 2006 (6 μg/ml). Cells were irradiated, washed, and cocultured with autologous CFSE-labeled CD3+ T cells in the absence or presence of CMV Ags for 8 d. Proliferation of CD8+ T cells was assessed by dilution of CFSE dye and by binding of PE-conjugated HLA-A2 NLVPMVATV tetramer complexes. A, Dot plots represent dilution of CFSE dye by proliferating T cells and tetramer staining from one of two representative experiments using blood of HLA-A*0201 donors. B, Percentages of proliferating CD8+ and CD4+ T cells (mean ± SD, n = 5). The comparisons were analyzed using the Wilcoxon rank test.