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. Author manuscript; available in PMC: 2012 Mar 1.
Published in final edited form as: Biol Blood Marrow Transplant. 2010 Oct 12;17(3):330–340. doi: 10.1016/j.bbmt.2010.09.020

Figure 3.

Figure 3

Role of host T cells in the GVL effect following pre-BMT vaccination with GM-MMB3.19 cells. B6 mice were treated with 0.2 ml of GK1.5 (1:6) and 2.43 (1:50) mAb against CD4+ and CD8+ T cells, respectively, 4 days prior to vaccination with a single s.c. dose of 2x105 GM-MMB3.19 cells. One week later (11 days after T cell-depletion), the mice were exposed to 8.5 Gy and 4 h later transplanted with either B6 syngeneic 2×106 ATBM cells alone (ATBM, MMB3.19 and GM-MMB3.19 pre-BMT no DL (T depleted host) groups] or in combination with 4-5×106 lymphocytes [DL, GM-MMB3.19 pre-BMT + DL, and GM-MMB3.19 pre-BMT + DL (T depleted host) groups]. On day 1 post-BMT, all groups except the ATBM control were challenged with an i.p. injection of 1×105 MMB3.19 cells. Data were pooled from 2-3 separate experiments consisting of 5-10 mice per group.