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. Author manuscript; available in PMC: 2012 Mar 1.
Published in final edited form as: Biol Blood Marrow Transplant. 2010 Oct 12;17(3):330–340. doi: 10.1016/j.bbmt.2010.09.020

Figure 4.

Figure 4

Percentage of CD11c+eGFP− cells in the skin-draining LN of vaccinated mice. B6 mice were vaccinated with a single dose of 2×105 GM-MMB3.19, non-GM-MMB3.19 cells or PBS alone. One week later, mice were exposed to 8.5 Gy and 4 h later transplanted with ATBM and lymphocytes from eGFP+B6 syngeneic mice in order to be able to differentiated between host (eGFP−) and donor cells (eGFP+). Three weeks post-BMT, cell suspensions from the skin-draining LN of individual mice were prepared for flow cytometric analysis to determine the percentage of CD11c+ expressing cells. Data were pooled from 2 separate experiments consisting of 3-6 mice per group. Statistical difference between groups was determined using Kruskal-Wallis one-way ANOVA analysis (p < 0.01) followed by Dunn’s multiple comparison test on individual pairs (*p < 0.05, ** p < 0.01).