Wild type and neur mutant embryos are double stained with Eve (Red) and Pdm1 (Green) at different times during GMC-1 development. While in wild type (panels A–I), the level of Pdm1 protein goes down from an early GMC-1 to a late GMC-1, in the mutant, Pdm1 is at a much higher level in an early GMC-1 (panels J, L) as well as in a mid or a late GMC-1 (panels M, O, P, R). The level of Eve is the same in a mid GMC-1 or a late GMC-1; an early GMC-1 has not yet started to express Eve (panels B and K). A late GMC-1 is larger than a mid or an early GMC-1, and a mid GMC-1 is smaller than an early GMC-1. In panel S, quantification of Pdm1 and Eve in GMC-1 was done by measuring the relative densities of Pdm1 and Eve staining signals in GMC-1.