Wild type (Panels A–C) and neur-GOF embryos (panels D–F) were subjected to heat shock at different time points during the development of the GMC-1 lineage (see text for details). In panels D and E, neur was induced prior to the formation of GMC-1, whereas in panel F, neur was induced prior to GMC-1 division, note the weak Eve expression in the RP2/sib pairs indicating their transformation to sib fate. Black arrowhead indicates a normal GMC-1, white arrowhead indicates either a GMC-1 with very weak Eve expression (panel D) or the location of a missing/eve-negative GMC-1 (panel E); large arrow indicates an RP2, small arrow indicates a sib.