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. Author manuscript; available in PMC: 2012 Apr 1.
Published in final edited form as: Neurobiol Dis. 2010 Dec 17;42(1):21–34. doi: 10.1016/j.nbd.2010.12.010

Figure 11.

Figure 11

Inhibition of CD47/SIRPα signal transduction decreases neutrophil diapedesis through CNS-derived EC monolayers. (A–C) Primary murine neutrophils were isolated, incubated for 15 min with either vehicle, an N-terminal SIRPα-blocking antibody, or the protein tyrosine kinase inhibitor genistein, and added to the upper chambers of a 5 µm-pore transwell filter containing a confluent monolayer of primary (P0-1) murine cortex-derived ECs previously incubated overnight with either vehicle (A), a CD47 blocking antibody (B), or the Gi heterotrimeric protein-blocking Pertussis toxin. (D) Incubation of neutrophils with the CD47-derived FG loop peptide (red squares) dose-dependently inhibited extravasation relative to control scrambled peptide (blue circles). After 1.5 hr, neutrophils in the lower chamber were fixed, stained with the PMN specific clone 7/4 (red) and Hoechst (blue), and eight 4× images from each treatment were counted. (B, inset) Hoechst stain reveals polymorphonuclear morphology of neutrophils (20×). Data are means ± SEM (n=3, ** p < 0.01).