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. Author manuscript; available in PMC: 2012 Apr 1.
Published in final edited form as: Neurobiol Dis. 2010 Dec 17;42(1):21–34. doi: 10.1016/j.nbd.2010.12.010

Figure 5.

Figure 5

The C-terminus of TSP-1, through CD47, decreases CNS EC proliferation. (A–C) Percent of Hoechst-stained nuclei (blue) of PECAM-expressing (green) WT EC monolayers positive for the proliferative marker Ki67 (red) were counted from at least six 20× images each from three independent experiments (cortex ECs, black bars; spinal cord ECs, white bars). A purified peptide of the CD47-binding TSP-1 C-terminal domain (E3T3C1) or functional blocking antibodies to TSP-1 (α-TSP-1 C-terminus) or CD47 (α-CD47) were incubated with ECs for 24 hours. Buffer control for spinal cord ECs was not determined (n.d.). Data are the means ± SEM (* p < 0.05).