Fig. 2. The effect of TGFβ-induced Tregs in GVHD.

OVA⁺ or OVA⁻ (B6 × bm12)F1 mice were lethally irradiated and transferred with 5 × 10⁶ TCD-BM alone or plus 1 × 10⁶ CD4⁺ T cells (Teffs) from B6 donors. OVA-specific iTregs (CD4⁺CD25⁺GFP⁺) were generated and purified by FACS sorting as shown in figure 1. OVA-specific iTregs or controls at 0.5 × 10⁶/mouse each were added into donor graft. Recipient survival (A) and body weight changes (B) are shown. Ten recipients were included in each group except that 5 mice were used in GFP⁺ or GFP− cells to OVA⁻ groups. The data are pooled from 2 replicate experiments using a similar setting.