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. Author manuscript; available in PMC: 2012 Mar 1.
Published in final edited form as: Dev Biol. 2011 Jan 5;351(1):110–119. doi: 10.1016/j.ydbio.2010.12.038

Fig. 5. Ascl2 gene expression in TS cells.

Fig. 5

A) Mouse TS cells; B) Rat TS cells; C) Normalized mouse and rat TS cell expression of Ascl2 mRNA. Mouse and rat TS cells were cultured under stem cell or differentiation conditions for the indicated days, harvested, and RNA extracted. qRT-PCR was used to estimate Ascl2 mRNA levels. Transcript levels were normalized to 18S RNA. Ratios of Ascl2 CT values to 18S CT values (mouse TS cell 18S CT value: 13.67 ± 0.058; rat TS cell 18S CT value: 13.63 ± 0.027) at peak expression in mouse TS cells: 1.67; and rat TS cells: 1.89. Bars represent means ± the standard error of the mean. Differentiation expression values significantly different from the stem cell level of expression are indicated with an asterisk (*P<0.05).