Representative redox western blots examining the Cr(VI)-mediated oxidation of Trx1, Trx2, and Prx3 in NHBE cells. A, redox status of cytosolic Trx1 after 6hr Cr(VI) treatment, and of mitochondrial Trx2 after 3 and 6 hr of treatment. For Trx2, the lanes marked DTT are isolated mitochondria that had been pre-treated with DTT which reduces Trx2 (Myers et al. 2008); these serve as a reference for the migration of reduced Trx2. B, redox status of mitochondrial Prx3 after 3 hr and 6 hr of treatment.