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. Author manuscript; available in PMC: 2012 Mar 15.
Published in final edited form as: Toxicology. 2011 Jan 13;281(1-3):37–47. doi: 10.1016/j.tox.2011.01.001

Fig. 7.

Fig. 7

Representative western blot to determine if TrxR nitration occurs as a result of Cr(VI) treatment. BEAS-2B cells were treated for 3 hr with the indicated concentrations of Cr(VI). The cells (ca. 1 ×107) were washed, harvested, and lysed and TrxR was immunoprecipitated from the lysates using anti-TrxR and protein A/G agarose as described in the methods. The immunoprecipitates were run on SDS-PAGE. The lanes that were probed with anti-nitrotyrosine (top) were loaded with 10-fold more of the immunoprecipitate than those that were probed with anti-TrxR (bottom). The top gel also includes nitrated BSA (1 µg) as a positive control, and BSA as a negative control.