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. 2001 Feb 1;29(3):743–752. doi: 10.1093/nar/29.3.743

Figure 5.

Figure 5

Figure 5

Substrate preference of Myh and Myh/Ape1. Substrate DNA (0.2 nM) was incubated with Myh alone or Myh/Ape1 at a constant molar ratio of 1:100. The glycosylase reaction was carried out at 37°C for 30 min. Then each reaction mixture, with alkaline treatment, was analyzed on a 15% denaturing polyacrylamide gel. An asterisk indicates the 5′-end-labeled strand. (A) Plot of the fraction of glycosylase product versus Myh. Each individual point is represented with error bars as an average of data from two to four experiments. (B) Plot of the fraction of glycosylase product versus Myh/Ape1 (only Myh concentrations are indicated; Ape1 concentrations can be calculated based on a constant 1:100 molar ratio).