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. Author manuscript; available in PMC: 2012 Mar 1.
Published in final edited form as: Dev Biol. 2010 Dec 23;351(1):62–69. doi: 10.1016/j.ydbio.2010.12.023

Fig. 3. Hand2 deletion in the SHF results in reduced number of cardiac progenitor cells in the pharyngeal region.

Fig. 3

Right lateral views of wild-type (A) or Islet1Cre:Hand2loxp/− (B) embryos at E9.5. Growth retardation of mutant embryos was noted. Transverse section from wild-type (C) or Islet1Cre:Hand2loxp/− (D) embryos at E9.5 demonstrated severe hypoplasia of right ventricle (rv) and a thin walled left ventricle in mutants, similar to Hand2-nulls. Compared with Islet1Cre:Hand2loxp/+;Rosa26LacZ embryo (E), LacZ staining was reduced in Islet1Cre:Hand2loxp/−;Rosa26LacZ (F), indicating a reduced number of cardiac progenitor cells in the SHF of this mutant. Results of TUNEL assay on wild type, Hand2−/−, and Islet1Cre:Hand2loxp/− E9.0 embryos in the pharyngeal mesoderm (G) and the outflow tract (H) are shown. Excessive cell death was observed in the pharyngeal mesoderm of Islet1Cre:Hand2loxp/− and Hand2−/− embryos, and in the outflow tract of Hand2−/− embryos. pa, pharyngeal arch; lv, left ventricle; a, atrium. *p = 0.05.