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. Author manuscript; available in PMC: 2011 Feb 15.
Published in final edited form as: J Neurochem. 2003 Jun;85(6):1513–1520. doi: 10.1046/j.1471-4159.2003.01793.x

Fig. 2.

Fig. 2

Characterization of human norepinephrine transporter (hNET)-myc and I155C. (a) Antibody specificity. Each panel represents a western blot of HeLa cells expressing either hNET, I155C or hNETmyc. The upper blot was visualized with anti-NET antibody and the lower blot with antibody against c-myc. (b) Sensitivity to inactivation. HeLa cells expressing either hNET (hNET wild-type), hNET-myc or I155C were incubated with either 100 μM cocaine or 2.5 mM [2-(trimethylammonium)ethyl]methanethiosulfonate (MTSET) for 10 min and then assayed for transport activity. All data plotted represent means ± SE, are based on triplicate determinations and are representative of three separate experiments. DA, Dopamine.