Fig. 4.

Quantitation of expressed human norepinephrine transporter (hNET) constructs. HeLa cells were transfected with mixtures of hNET-myc and I155C cDNA such that the total DNA per well was constant; however, the percent of hNET-myc in the mixture varied from 0 to 100% as indicated. Extracts of the cells were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and were visualized by western blot analysis using (a) anti-myc and (b) anti-NET antibodies. The relative amount of each hNET construct was estimated by comparison with standard curves using cells expressing either hNET-myc or I155C. The values are expressed in terms of the amount of cell protein required in the standard curve to give an equivalent signal.