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. Author manuscript; available in PMC: 2011 Feb 15.
Published in final edited form as: Mol Immunol. 2010 Nov–Dec;48(1-3):59–72. doi: 10.1016/j.molimm.2010.09.012

Table 3.

Kinetic constants and affinities of various rFab with immobilized biotin-Aβ1–16 (bAβ1–16) and biotinylated Aβ1–42 HMW oligomers (bAβ1–42-HMW oligomers).

Analyte Ligand ka (×105 M−1 s−1) kd (×10−3 s−1) KD (nM)
WO2 rFab bAβ1–16 3.65 1.02 2.7
20.1 rFab bAβ1–16 4.6 1.4 3.0
20.1_VH/WO2_VL rFab bAβ1–16 0.76 0.65 8.5
WO2_VH/20.1_VL rFab bAβ1–16 1.44 17.8 123.8
WO2/20.1_HCDR3 rFab bAβ1–16 3.57 23.9 66.9
WO2/10D5_HCDR3 rFab bAβ1–16 0.63 9.74 153.9
WO2/12A11_HCDR3 rFab bAβ1–16 1.71 44.9 262.5
WO2/A9_HCDR3 rFab bAβ1–16 0.08 33.4 4200
WO2/12B4_HCDR3 rFab bAβ1–16 0.01 69 6500
20.1/10D5_HCDR3 rFab bAβ1–16 0.62 5.45 87.9
gWO2_VH/WO2_VL rFab bAβ1–16 1.07 17.1 162
WO2_VH/gWO2_VL rFab bAβ1–16 1.52 35.1 231
WO2 rFab bAβ1–42-HMW oligomers 3.65 0.89 2.3
20.1 rFab bAβ1–42-HMW oligomers 2.29 0.63 2.8
20.1_VH/WO2_VL rFab bAβ1–42-HMW oligomers 0.63 0.57 9.0
WO2_VH/20.1_VL rFab bAβ1–42-HMW oligomers 2.19 12.7 58.1
WO2/20.1_HCDR3 rFab bAβ1–42-HMW oligomers 3.18 11.2 35.28
WO2/10D5_HCDR3 rFab bAβ1–42-HMW oligomers 0.61 5.46 89.24
WO2/12A11_HCDR3 rFab bAβ1–42-HMW oligomers 1.33 26.1 135.3
WO2/A9_HCDR3 rFab bAβ1–42-HMW oligomers nd nd nd
WO2/12B4_HCDR3 rFab bAβ1–42-HMW oligomers nd nd nd
20.1/10D5_HCDR3 rFab bAβ1–42-HMW oligomers 0.74 4.67 62.7
gWO2_VH/WO2_VL rFab bAβ1–42-HMW oligomer 1.30 15.7 121
WO2_VH/gWO2_VL rFab bAβ1–42-HMW oligomer 2.18 28.5 131