FIGURE 8.

Both compounds increase translation of a luciferase reporter fused to the small, endogenous uORF from PRE2 beginning with a UUG codon. (A) Schematic of the reporter. As a control, the uORF was fused out of frame from the luciferase coding region. BY4741 expressing the reporter was treated with NSC218351 (B) or NSC92218 (C), and Fluc activity was measured. Closed squares are the in-frame reporters, and open squares are the out-of-frame controls. Fluc activity with DMSO alone was used to normalize the activity with compound. Points are averages of two independent experiments ± average deviation.