FIGURE 6.

Analysis of sex-specific splicing in germline-less tudor mutant heads. (A) Alternative splicing of classical sex determination regulatory genes is not affected in germline-less (tud) fly heads. Patterns of expression and sex-specific isoforms were analyzed as in Figure 3 for the indicated genes in RNA preparations from wild-type (wt) and tud mutant female and male fly heads. (B) Alternative splicing of additional sex-specific splicing differences between female and male in adult fly heads of wild-type and germline-less (tud) flies. Results from six genes are represented as fold changes between female and male flies. (**) Statistically significant differences, reproducibly observed in more than three biological replicas.