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Tertiary interactions mapped by UV crosslinking. Mapping of the tL.1: 4SU-tD.1 crosslinks. Each crosslinked species (1, 2, 3, and 4) was gel-purified and partially alkaline hydrolyzed (see Supplemental Fig. S7). The crosslinked residues were then mapped using a PAGE sequencing gel with T1 and hydrolysis ladders as references.
