FIGURE 2.

(A) Sedimentation coefficient distribution c(s) determined by program Sedfit for three different Hfq₆ concentrations, 3.2, 5.7, and 12 μM shown as dotted, dashed, and solid lines, respectively. The average integrated value for these data is 3.51 ± 0.03 S. (B) Sedimentation coefficient distribution displayed as normalized g(s) for Hfq₆ at 6.9 μM alone as solid line (average integrated value for Hfq data is 3.42 ± 0.04 S), and with 4.4 μM and 8.3 μM FAM-A₁₈ added. Upper pair of overlapping dashed and dotted lines show the 6.9:4.4 mixture evaluated by absorbance at 274 nm and 495 nm, respectively. Lower pair of dashed and dotted lines display the 6.9:8.3 mixture evaluated at the same two wavelengths. Lack of alignment of c(s) and g(s) peaks for Hfq₆ alone (3.51 S vs. 3.42 S) is attributed to minor components affecting the main c(s) peak.