FIGURE 4.

The in vivo decapping rate of RPL41A mRNA was determined using qSL-RT-PCR assay. The half-life of RPL41A was measured in wild-type yeast (A) and in a strain wherein the XRN1 gene is deleted (Δxrn1) (B). (C) Decapped RPL41A mRNA was measured using the qSL-RT-PCR assay in the Δxrn1 strain following transcription shutoff. Relative amount of decapped RNA was calculated after normalization to total RPL41A at each time-point, relative to time = 0. (D) The rate of decapping was determined by linear regression analysis of the data in the graph in C with linear reaction kinetics between 5 and 30 min. The slope of the line, the reaction rate, is shown along with error and correlation coefficient. In all graphs, the mean value of the replicates is plotted and standard error is indicated above and below the data points.