FIGURE 2.

Growth inhibition of S. cerevisiae cells by TOP2 M1 3/4EGS RNA. (A) Phenotype investigation of M1 3/4EGSs transformants on plates. Cells were transformed with empty vector pYES2 or plasmids (M1T12 and M1T28) containing the galactose-inducible GAL1 promoter. After growth to saturation in SC liquid medium, cells were serially diluted (1/10, 1/100, 1/1000, and 1/10,000), and spotted onto SC plates containing 2% glucose (to repress expression of M1 3/4EGS) or galactose (to induce transcription). The results are shown after 24 and 36 h of incubation at 30°C. At least three independent experiments were carried out and a representative result is shown. (B) Growth profile of M1 3/4EGSs transformants in liquid medium. Cells with the indicated plasmids were incubated in SC liquid medium at 30°C to saturation, and then diluted the saturated culture to OD₅₆₀ = 0.1 with SC medium containing 2% glucose or galactose. The OD₅₆₀ values of each culture were measured every 6 h over 2 d. Values presented here represent means of three independent incubations of the same preparation. M1T12 and M1T28 transformants in galactose medium (triangles and pluses).