Table 2.
Transepithelial resistance data before and after particle addition.
Transepithelial resistance (Ω · cm2) | |||||
---|---|---|---|---|---|
Treatment group | Raw data for Control/TER | Raw data for post-treatment/TER | Delta changes after pre-treatment | Raw data for post-particle/TER | Delta changes after particle exposure |
Particle-only groups | |||||
Particle/only-5 | 451 ± 19 | – | – | 580 ± 25 | 134 ± 7# |
Particle/only-60 | 451 ± 19 | – | – | 484 ± 31 | 27 ± 12P/O-5 |
24 h Caco-2/macrophage groups | |||||
Macrophage/particle-5 | Control/TER subset | 144 ± 12 | −438 ± 31# | 213 ± 6 | 77 ± 6† |
Macrophage/particle-60 | Control/TER subset | 144 ± 12 | −438 ± 31# | 206 ± 15 | 56 ± 4 |
24 h macrophage-conditioned medium groups | |||||
Macrophage-conditioned medium/particle-5 | Control/TER subset | 314 ± 31 | −221 ± 41#C/M | 450 ± 41 | 125 ± 11P/O-5,M/P-5 |
Macrophage-conditioned medium/particle-60 | Control/TER subset | 314 ± 31 | −221 ± 41#C/M | 346 ± 53 | 43 ± 11 |
24 h untreated-medium groups | |||||
Untreated-medium/particle-5 | Control/TER subset | 566 ± 10 | 31 ± 10M-CM | 736 ± 15 | 174 ± 12†P/O-5,M-CM/P-5 |
Untreated-medium/particle-60 | Control/TER subset | 566 ± 10 | 31 ± 10M-CM | 639 ± 20 | 68 ± 10† |
Mean transepithelial resistance (TER) values ± standard error of the mean (SEM) for n ≥ 21 samples are calculated from a minimum of two experiments. Pre-treatment delta changes (italics) in TER following treatments are calculated well by well as [pre-treatment/TER − mean control/TER from the same experiment]. Particle associated delta changes (italics) in TER between treatment and particle exposure are calculated well by well as [post-particle/TER − post-treatment/TER]. Statistical differences (P ≤ 0.05) for delta changes are illustrated as ‘#’ a difference from control/TER, ‘†’ from post-treatment/TER, ‘C/M’ from the Caco-2/macrophage effect, ‘P/O-5’, ‘M/P-5’ and ‘M-CM/P-5’ from particle/only-5, macrophage/particle-5 and macrophage-conditioned medium/particle-5 respectively.